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Figure 3. Muse cell sorting and confirmation of the pluripotency. (A) Venus-Akaluc-labeled cells were sorted. SSEA-positive cells were sorted as Muse cells and SSEA-negative cells were sorted as non-Muse cells. (B) M-cluster formation in single-cell suspension. SSEA = stage-specific embryonic antigen; -A = -Area; SSC = side scatter; FITC = fluorescein isothiocyanate; APC = <t>allophycocyanin.</t>
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Figure 3. Muse cell sorting and confirmation of the pluripotency. (A) Venus-Akaluc-labeled cells were sorted. SSEA-positive cells were sorted as Muse cells and SSEA-negative cells were sorted as non-Muse cells. (B) M-cluster formation in single-cell suspension. SSEA = stage-specific embryonic antigen; -A = -Area; SSC = side scatter; FITC = fluorescein isothiocyanate; APC = allophycocyanin.

Journal: Cartilage

Article Title: Investigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing.

doi: 10.1177/19476035241262020

Figure Lengend Snippet: Figure 3. Muse cell sorting and confirmation of the pluripotency. (A) Venus-Akaluc-labeled cells were sorted. SSEA-positive cells were sorted as Muse cells and SSEA-negative cells were sorted as non-Muse cells. (B) M-cluster formation in single-cell suspension. SSEA = stage-specific embryonic antigen; -A = -Area; SSC = side scatter; FITC = fluorescein isothiocyanate; APC = allophycocyanin.

Article Snippet: MSCs were first incubated with rat anti-SSEA-3 IgM antibody (clone MC-631; Millipore, Billerica, MA) and further incubated with an Allophycocyanin AffiniPure F(ab’)2 Fragment Goat Anti-Rat IgM (immunoglobulin M) antibody (Jackson ImmunoResearch, West Grove, PA).

Techniques: FACS, Labeling, Suspension